Authors:Devi, W.S; Sharma, G.J
Abstract:An efficient protocol for in vitro propagation of Melocanna bambusoides Trin. is reported. Single nodal segments obtained from mature field grown culm collected during monsoon were tested for bud-break and shoot growth on Murashige and Skoog (MS) medium supplemented with different concentrations of 6- benzylaminopurine (BAP). The early bud-break was obtained on 2-5 mg l-1 BAP with 1-3 shoots per explants. Axillary shoots produced were multiplied on MS medium supplemented with 3-5 mg l-1 BAP in liquid medium. Combination of 2 mg l-1 kinetin (Kn) with 2 mg l-1 BAP enhanced the multiplication rate. In vitro rooting was carried out in half-strength MS medium. Addition of optimum concentration of 10 mg l-1coumarin to 3 mg l-1 3- indoleacetic acid (IAA) and 3 mg l-1 3- indolebutyric acid (IBA) enhanced the rooting percentage. More than 80 per cent of the shoots could be rooted in halfstrength MS medium containing 3 mg l-1 IBA, 10 mg l-1 coumarin and 0.05 mg l-1 BAP before transferring them to a semi-solid rooting medium. Synergistic interaction between an optimum concentration of 10 mg l-1 coumarin with auxins stimulates root growth and development. After a period of in vitro hardening, the propagules were acclimatized and then transferred to the soil with more than 80 per cent success
Keywords:auxins; benzyladenine; buds; explants; growth; IAA; IBA; in vitro culture; in vitro reg