Journal of Bamboo and Rattan

DNA barcoding: An emerging tool for precise identification and certification of planting stock in taxonomically challenging bamboo species

Authors

  • K. Sijimol

    Author
  • Suma Arun Dev

    Scientist
    Author
  • E. M. Muralidharan

    Scientist
    Author
  • V. B. Sreekumar

    Scientist
    Author

DOI:

https://doi.org/10.55899/

Keywords:

Bamboo, taxonomy, species identification, certification, DNA barcoding

Abstract

Bamboos are a group of woody arborescent grasses that provides for the livelihood of a large number of people living in rural areas in many parts of the world. Owing to the increased demand for bamboos for traditional as well as new commercial uses, there is an urgent need for undertaking plantation of the commercially important bamboo species. To ensure higher productivity it is also important that the plantations be established using superior quality planting materials. From the taxonomic standpoint, bamboos are a challenging group of plants. Species identification in bamboos is mainly based on morphological characters and the unpredictable flowering behavior coupled with environmental plasticity leads to taxonomic complexities. This is particularly confounding when the plants are in the juvenile stage as in the bamboo nurseries. This poses certain hurdles in the certification of superior planting materials owing to the lack of distinguishable morphological characters. Mixing up of species with similar morphological features is rather common in bamboo nurseries and mistakes are realized only when the plants attain maturity after few years. Hence a well defined molecular tool like DNA barcoding is envisaged to tackle these taxonomic complexities in species identification as well as for the certification of planting materials. This article brings out the significance of DNA barcoding in species identification and to resolve taxonomic complexities pertaining to bamboo. The ongoing research in the use of DNA markers in taxonomy and biosystematics of bamboo and the challenges faced is also reviewed. 

Author Biographies

  • K. Sijimol

    Kerala Forest Research Institute, Peechi, Thrissur, Kerala-680653, India

  • Suma Arun Dev, Scientist

     Kerala Forest Research Institute, Peechi, Thrissur, Kerala-680653, India

  • E. M. Muralidharan, Scientist

     Kerala Forest Research Institute, Peechi, Thrissur, Kerala-680653, India

  • V. B. Sreekumar, Scientist

     Kerala Forest Research Institute, Peechi, Thrissur, Kerala-680653, India

References

Anbazhagan, M., Elayaraja, B., Sudharson, S., Balachandran, B. and Arumugam, K. 2014. dentification of Ocimum species through DNA barcodes. International Journal of Current Science. 13: 127-137.

Anvarkhah, S., Hosseini, M.K., Hassan Rashed, M. M., Panah, A.D.E. and Hashemi, H. 2013. Identification of three species of genus Allium using DNA barcoding. International Journal of Agriculture and Crop Sciences. 5: 1195 1203.

Attigala, L., Kathriarachchi, H. S. and Clark, L.G. 2016. Taxonomic Revision of the Temperate Woody Bamboo Genus Kuruna (Poaceae: Bambusoideae: Arundinarieae). Systematic Botany. 41(1):174-196.

Awomukwu, D. A., Nyananyo, B.L., Spies, P. and izani, B.L. 2015. Identification, Validation and classification of the genus Phyllanthus in Nigeria using ITS genetic marker and the taxonomic implication. International Journal of Genetics and Genomics. 3(1): 1-7.

Bahadur, K.N. and Jain, S.S. 1981. Rare bamboos in India. Indian Journal of Forestry. 4(4): 280-286.

Bamboo Phylogeny Group, 2012. An updated tribal and subtribal classification of the bamboos (Poaceae: Bambusoideae). In: Proceedings of the 9th World Bamboo Congress, 10–12 April 2012, Antwerp, Belgium, pp. 3–27.

Bennet, S.S.R. and Gaur, R.C. 1990. Thirty seven bamboos growing in India. Forest Research Institute, Dehra Dun.

Bieniek, W., Mizianty, M. and Szklarczyk, M. 2015. Sequence variation at the three chloroplast loci (matK, rbcL, trnH-psbA) in the Triticeae tribe (Poaceae): comments on the relationships and utility in DNA barcoding of selected species. Plant Systematics and Evolution. 301: 1275–1286.

Birch, J. L., Cantrill, D. J., Walsh, N.G. and Murphy, D. J. 2014. Phylogenetic investigation and divergence dating of Poa (Poaceae: tribe Poeae) in the Australasian Region. Botanical Journal of the Linnean Society. 175: 523–552.

Biswal, D.K., Debnath, M., Kumar, S. and Tandon, P. 2012. Phylogenetic reconstruction in the Order Nymphaeales: ITS2 secondary structure analysis and in silico testing of maturase k (matK) as a potential marker for DNA barcoding. BMC Bioinformatics. 13:S26.

Buchheim, M.A., Keller, A., Koetschan, C., Forster, F., Merget, B. and Wolf, M. 2011. Internal Transcribed Spacer 2 (nu ITS2 rRNA) Sequence-Structure Phylogenetics: Towards an Automated Reconstruction of the Green Algal Tree of Life. PLoS One. 6(2): e16931.

Bystriakova, N., Kapos, V., Lysenko, I. and Stapleton, C. 2003. Distribution and conservation status of forest bamboo biodiversity in the Asia‐Pacific region. Biodiversity and Conservation. 12: 1833–1841.

Cai, Z.M., Zhang, Y.X., Zhang, L.N., Gao, L.M. and Li, D.H. 2012. Testing four candidate barcoding markers in temperate woody bamboos (Poaceae: Bambusoideae). Journal of Systematics and Evolution. 50 (6): 527-539.

Castro, C., Hernandez, A., Alvarado, L. and Flores, D. 2015. DNA Barcodes in Fig Cultivars (Ficus carica L.) Using ITS Regions of Ribosomal DNA, the psbA trnH Spacer and the matK Coding Sequence. American Journal of Plant Sciences. 6: 95-102.

CBOL Plant Working Group, 2009. DNA barcoding in land plants. PNAS. 106: 12794–12797.

Chao, C.S. and Renvoize, S.A. 1989. Revision of species described under Arundinaria (Gramineae) in South East Asia and Africa. Kew Bulletin. 44: 349-367. Chase, M.W., Cowan, R.S., Hollingsworth, P.M., van den Berg, C. and Madrinan, S. 2007. A proposal for a standardised protocol to barcode all land plants. Taxon. 56: 295–299

Clark, L.G., Dransfield, S., Triplett, J. and Sanchez-Ken, J.G. 2007. Phylogenetic relationships among the one-flowered, determinate genera of Bambuseae (Poaceae: Bambusoideae). Aliso. 23: 315–332.

Clark, L.G., Zhang, W. and Wendel, J.F. 1995. A phylogeny of the grass family (Poaceae) based on ndhF sequence data. Systematic Botany. 20: 436–460.

Clement, W.L. and Donoghue, M.J. 2012. Barcoding success as a function of phylogenetic relatedness in Viburnum, a clade of woody angiosperms. BMC Evolutionary Biology. 12(1): 73-77.

Collins, R.A. and Cruickshank, R. H. 2012. The seven deadly sins of DNA barcoding. Molecular Ecology Resources. 13(6): 969-975.

Das, M.M., Mahadani, P., Singh, R., Karmakar, K. and Ghosh, S.K. 2013. matK sequence based plant DNA barcoding failed to identify Bambusa (Family: Poaceae) species from Northeast India. Journal of Environment and Sociobiology. 10(1): 49-54.

de Groot, G.A., During, H.J., Maas, J.W., Schneider, H. and Vogel, J.C. et al. 2011. Use of rbcL and trnL-F as a two-Locus DNA Barcode for identification of NW European Ferns: An ecological perspective. PLoS One. 6(1): e16371

Ding, Y.L. and Zhao, Q.S. 1994. Studies on the comparative anatomy of bamboo leaves and its significance for bamboo systematic taxonomy. Journal of Nanjing Forestry University. 18 (3): 1-6.

Dransfield, S. 1980. Bamboo taxonomy in the Indo-Malayan region. Bamboo Research in Asia. Proceedings of a Workshop, 28-30 May 1980, Singapore. IDRC, Ottawa: 121- 130.

Drumwright, A.M., Allen, B.W., Huff, K.A., Ritchey, P.A. and Cahoon, A.B. 2011. Survey and DNA Barcoding of Poaceae in Flat Rock Cedar Glades and Barrens State Natural Area, Murfreesboro, Tennessee. Castanea. 76(3): 300 310.

Ebihara, A., Nitta, J.H. and Ito, M. 2010. Molecular Species Identification with Rich Floristic Sampling: DNA Barcoding the pteridophyte flora of Japan. PLoS One. 5(12): e15136.

Eevera, T., Rajandran, K., Saradha, S. and Lashmi, A. 2008. Analysis of genetic variation in selected bamboo species. Tree and Forestry Science and Biotechnology. 2(1): 54-56.

Ennos, R.A., French, G.C. and Hollingsworth, P.M. 2005. Conserving taxonomic complexity. Trends in Ecology and Evolution. 20: 164–168.

Friar, E. and Kochert, G. 1991. Bamboo germplasm screening with nuclear restriction fragment length polymorphisms. Theoretical and Applied Genetics. 82: 697–703.

Gamble, J.S. 1896. The Bambuseae of British India. Annals of the Royal Botanic Garden. 7(1): 1-133. Ghahramanzadeh, R., Esselink, G., Kodde, L.P., Duistermaat, H., Van Valkenburg, J. L.C.H., Marashi, S. H., Smulders, M. J. M. and Van De Wiel, C. C. M. 2013.

Efficient distinction of invasive aquatic plant species from non-invasive related species using DNA barcoding. Molecular Ecology Resources. 13: 21–31.

Gielis, J., Everaert, I. and De Loose, M. 1997. Analysis of genetic variability and relationships in Phyllostachys using random amplified polymorphic DNA. In: Chapman G (Ed.) The Bamboos, Academic Press, London. 107-124.

Goyal, A.K., Kar, Pallab. And Sen, A. 2013. Advancement of bamboo taxonomy in the era of molecular biology: a review. In: Arnab S (Ed.) Biology of useful plants and microbes, Narosa Publishing House, New Delhi, India. 107-208.

Hebert, P.D.N., Cywinska, A., Ball, S.L. and deWaard, J.R. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society Biological Sciences. 270: 313–321.

Hollingsworth, M.L., Clark, A.A., Forrest, L.L., Richardson, J. and Pennington, R.T. 2009. Selecting barcoding loci for plants: evaluation of seven candidate loci with species-level sampling in three divergent groups of land plants. Molecular Ecology Resources. 9: 439–457.

Hollingsworth, P.M., Graham, S.W. and Little, D.P. 2011. Choosing and using a plant DNA barcode. PLoS One. 6: e19254.

Hsiao, L.Y. and Rieseberg, L.H. 1994. Population genetic structure of Yushania niitakayamensis (Bambusoideae, Poaceae) in Taiwan. Molecular Ecology. 3: 201-208.

Huang X. C., Ci, X.Q., Conran, J.G. and Li, J. 2015. Application of DNA Barcodes in Asian Tropical Trees – A Case Study from Xishuangbanna Nature Reserve, Southwest China. PLoS One. 10(6): e0129295.

Ito, Y., Tanaka, N., Pooma, R. and Tanaka, N. 2014. DNA barcoding reveals a new record of Potamogeton distinctus (Potamogetonaceae) and its natural hybrids, P. distinctus × P. nodosus and P. distinctus × P. wrightii ( P. malainoides) from Myanmar. Biodiversity Data Journal. 2: e1073.

Jethra, G., Mishra, A.K., Pandey, P.S., Choudhary, S. and Chandrasekharan, H. 2014. Phylogenetic and structural scrutiny of matK gene from wheat representing Poaceae family for DNA barcoding. International Journal of Science and Nature. 5 (1): 141-146.

Kelchner, S.A. and Bamboo Phylogeny Group. 2013. Higher level phylogenetic relationships within the bamboos (Poaceae: Bambusoideae) based on five plastid markers. Molecular Phylogenetics and Evolution. 67(2): 404-413.

Kelchner, S.A. and Clark, L.G. 1997. Molecular evolution and phylogenetic utility of the rpl16 intron in Chusquea and the Bambusoideae (Poaceae). Molecular Phylogenetics and Evolution. 8: 385–397.

Kress, W.J. and Erickson, D.L. 2007. A two-locus global DNA barcode for land plants: The coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS One. 2: e508.

Kumar, M. 1995. A re-investigation on the taxonomy of the genus Ochlandra Thw. (Poaceae: Bambusoideae). Rheedea. 5: 63-89

Kumar, M. 2011. Bamboos of Peninsular India: All India Coordinated Project on Taxonomy (AICOPTAX): Grasses and Bamboos Part-II. Art Options, New Delhi.

Kumar, M. and Remesh, M. 2008. Munrochloa, A new genus (Poaceae; Bambusoideae) with a new combination from India. Journal of Botanical Research. 2(1): 373-378.

Kumar, M., Remesh, M. and Sequiera, S. 2001. Ochlandra keralensis (Poaceae- Bambusoideae) - A new reed-bamboo from Southern Western Ghats, India. Journal of Economic and Taxonomic Botany. 25: 49-51.

Kumar, M., Seethalakshmi, K.K. and Sequiera, S. 1999. Two new species of Ochlandra Thw. (Poaceae - Bambusoideae) from Southern India. Rheedea. 9: 31- 35.

Lee, H.W. and Kim, M.S. 2015. Species delimitation in the green algal genus Codium (Bryopsidales) from Korea using DNA barcoding. Acta Oceanologia Sinica. 34 (4): 114-124.

Les, D.H., Moody, M.L., Jacobs, S.W.L. and Bayer, R.J., 2002. Systematics of seagrasses (Zosteraceae) in Australia and New Zealand. Systematic Botany. 27: 468–484.

Li, Y., Gao, L.M., Poudel, R.C., Li, D.H. and Forrest, A. 2011. High universality of matK primers for barcoding gymnosperms. Journal of Systematics and Evolution. 49 (3): 169–175.

Li, F.W., Tan, B., Buchbender, V., Moran, R. and Rouhan, G. et al. 2009. Identifying a mysterious aquatic fern gametophyte. Plant Systematics and Evolution. 281: 77–86.

Lin, Y., Lu, J.J., Wu, M.D., Zhu, M.B., Fang, W., Ide, Y. and Tang, Q. D. 2014. Identification, cross-taxon transferability and application of full-length cDNA SSR markers in Phyllostachys pubescens. Springer Plus. 3: 486.

Liu, J., Yan, H.F., Newmaster, S.G., Pei, N., Ragupathy, S. and Ge, X.J. 2014. The use of DNA barcoding as a tool for the conservation biogeography of subtropical forests in China Biodiversity Research. Diversity and Distributions: 21 (2): 188-199.

Liu, Y., Yan, H.F., Cao, T. and Ge, X.J. 2010. Evaluation of 10 plant barcodes in Bryophyta (Mosses). Journal of Systematics and Evolution. 48 (1): 36–46.

Liu, J., Zhou, S., Li, Z., Dong, W. and Yang, R. 2014. DNA barcoding of Leymus (Poaceae). Journal of Research in Agriculture and Animal Science. 2(2): 1-11.

Loh, J.P., Kiew, R., Set, O., Gan, L.H. and Gan, Y.Y. 2000. A study of genetic variation and relationships within the bamboo subtribe using Amplified Fragment Length Polymorphism. Annals of Botany. 85: 607-612.

Lopez-Alvarez, D., Lopez-Herranz, M.L., Betekhtin, A. and Catalan, P. 2012. A DNA barcoding method to discriminate between the model plant Brachypodium distachyon and its close relatives B. stacei and B. hybridum (Poaceae). PLoS One. 7(12): e51058.

Lucas, C., Thangaradjou, T. and Papenbrock, J. 2012. Development of a DNA Barcoding System for Seagrasses: Successful but Not Simple. PLoS One. 7(1): e29987.

Ma, X.Y. Xie, C.X., Song, J.Y., Yao, H., Luo, K., Zhu, Y.J., Gao, T., Pang, X.H., Qian, J. and Chen, S.L. 2010. Species identification of medicinal pteridophytes by a DNA barcode marker, the chloroplast psbA-trnH intergenic region. Biological and Pharmaceutical Bulletin. 33(11): 1919-1924.

Majumdar, R.B. 1989. In: Karthikeyan S., Jain S.K., Nayar M.P. and Sanjappa M. Flora Indicae, Enumeratio Monocotyledonae. Botanical Survey of India, Howrah, Calcutta: 274-283.

Marulanda, M.L., Lopez, A.M. and Claroz, J.L. 2007. Analyzing the genetic diversity of Guadua spp. in Colombia using rise and sugar cane microsatellites. Crop Breeding and Applied Biotechnology. 7: 43-51.

Marulanda, M.L., Marquez, P. and Londono, X. 2002. AFLP analysis of Guadua angustifolia (Poaceae: Bambusoideae) in Colombia with emphasis on the Coffee Region. Bamboo Science and Culture: The Journal of the American Bamboo Society. 16(1): 32-42.

McClure, F. A. 1966. The Bamboos: A Fresh Perspective. Harvard University Press, Cambridge, Mass, USA. 347pp.

Mukherjee, A.K., Panda, Ratha, S. S., Dhar, S., Debata, A.K., Acharya, P.K., Mandal, S., C.P. and Mahapatra, A.K. 2010. Genetic relationships among 22 taxa of bamboo revealed by ISSR and EST-based random primers. Biochemical Genetics. 48 (11): 1015-1025.

Munro, W. 1868. A monograph of the Bambuseae. Transactions of the Linnean Society of London. 26: 1-157.

Nag, A., Gupta, P., Sharma, V., Sood, A., Ahuja, S.P. and Sharma, K.R. 2013. AFLP and RAPD based genetic diversity assessment of industrially important reed bamboo (Ochlandra travancorica Benth). Journal of Plant Biochemistry and Biotechnology. 22: 144-149.

Nakai, T. 1925. The new genera of Bambusaceae with special remarks on the related genera growing in eastern Asia. Journal of the Arnold Arboretum. 6(3): 145 -153.

Nayak, S. and Rout, G.R. 2005. Isolation and characterization of microsatellites in Bambusa arundinacea and cross species amplification in other bamboos. African Journal of Biotechnology. 4 (2): 151-156.

Nayak, S., Rout, G.R. and Das, P. 2003. Evaluation of the genetic variability in bamboo using RAPD markers. Plant Soil and Environment. 49(1): 24–28.

Newmaster, S.G., Fazekas, A.J. and Ragupathy, S. 2006. DNA barcoding in land plants: evaluation of rbcL in a multigene tiered approach. Canadian Journal of Botany. 84: 335–341.

Nitta, J.H. 2008. Exploring the utility of three plastid loci for biocoding the filmy ferns (Hymenophyllaceae) of Moorea. Taxon. 57: 725–736.

Orhnberger, D. and Georrings, J. 1986. The Bamboos of the World. International Book Publishers, Dehra Dun, India.

Palhares, R.M., Drummond, M.G., dos Santos Alves Figueiredo Brasil B., Cosenza, G.P., das Gracas Lins Brandao M. and Oliveira, G. 2015. Medicinal plants recommended by the World Health Organization: DNA Barcode Identification Associated with chemical analyses guarantees their quality. PLoS One. 10(5): e0127866.

Ramanayakae, S.M.S.D., Meemaduma, V.N. and Weerawardhanae, T.E. 2006. Genetic diversity and relationships within populations of Dendrocalamus giganteus Wall ex Munro and Ochlandra stridula Moon ex Thwaites in Sri Lanka using RAPD markers. Journal of Bamboo and Rattan. 5: 141-149.

Sass, C., Little, D.P., Stevenson, D.W. and Specht, C.D. 2007. DNA barcoding in the Cycadales: testing the potential of proposed barcoding markers for species identification of Cycads. PLoS One. 2: e1154.

Seethalakshmi, K.K. and Kumar, M. 1998. Bamboos of India: A Compendium. Kerala Forest Research Institute, Peechi and International Network for Bamboo and Rattan, Beijing.

Shalini, A., Meena, R.K., Tarafdar, S. and Thakur, S. 2013. Evaluation of genetic diversity in bamboo through DNA marker and study of association with morphological traits. Bulletin of Environment, Pharmacology and Life Sciences. 2 (8): 78-83.

Sharma, M.L. 1996. The genus Oxytenanthera in Asia. Indian Forester. 122(2): 187-188.

Sharma, R.K., Gupta, P., Sharma, V., Sood, A., Mohapatra, T. and Ahuja, P.S. 2008. Evaluation of rice and sugarcane SSR markers for phylogenetic and genetic diversity analyses in bamboo. Genome. 51(2):91-103.

Soderstrom, T.R. and Ellis, R.P. 1987. The position of bamboo genera and allies in a system of grass classification. Grass systematics and evolution. Smithsonian Press, Washington: 225-233.

Sosa, V., Saules, M.T., Cuellar, M.A. and Vovides, A.P. 2013. DNA barcoding in endangered Meso American groups of plants. Botanical Review. 79(4): 469-482.

Sungkaew, S., Stapleton, S.M.A., Salamin, N. and Hodkinson, T.R. 2009. Non monophyly of the woody bamboos (Bambuseae; Poaceae): a multi-gene region phylogenetic analysis of Bambusoideae s.s. Journal of Plant Research. 122: 95–108.

Techen, N., Parveen, I., Pan, Z. and Khan, I.A. 2014. DNA barcoding of medicinal plant material for identification. Current Opinion in Biotechnology. 25: 103–110.

Tewari, D.N. 1992. A Monograph on Bamboo. International Book Distributors, Dehra Dun: 152-156

Triplett, J.K. and Clark, L.G. 2010. Phylogeny of the temperate woody bamboos (Poaceae: Bambusoideae) with an emphasis on Arundinaria and allies. Systematic Botany. 35: 102–120.

Trivedi, S., Aloufi, A. A., Ansari, A. A. and Ghosh, S.K. 2015. Role of DNA barcoding in marine biodiversity assessment and conservation: An update. Saudi Journal of Biological Sciences. 23(2): 161-171.

Uchimura, M., Faye, E.J., Shimada, S., Inoue, T. and Nakamura, Y. 2008. A reassessment of Halophila species (Hydrocharitaceae) diversity with special reference to Japanese representatives. Botanical Markers. 51: 258–268.

Unnikrishnan, P. 2003. Taxonomic studies on the bamboos of South India, PhD Thesis, University of Calicut.

Usui, H. 1957. Morphological studies on the prophyll of Japanese bamboos. Tokyo Botany Magazine. 70: 223–227.

Vivas, C.V. and Moraes, R.C.S. 2014. DNA barcoding in Atlantic Forest plants: What is the best marker for Sapotaceae species identification?. Genetics and Molecular Biology. 37( 4): 662-670.

Wang, W., Wu, Y., Yan, Y., Ermakova, M., Kerstetter, R. and Messing, J. 2010. DNA barcoding of the Lemnaceae, a family of aquatic monocots. BMC Plant Biology. 10: 205-216.

Watanabe, M., Ito, M. and Kurita, S. 1994. Chloroplast DNA phylogeny of Asian bamboos (Bambusoideae, Poaceae) and its systematic implication. Journal of Plant Research. 107: 253–261.

Waycott, M., Duarte, C.M., Carruthers, T.J.B., et al. 2009. Accelerating loss of seagrasses across the globe threatens coastal ecosystems. PNAS. 106: 12377–12381.

Xu, S., Li, D., Li, J., Xiang, X., Jin, W., Huang, et al. 2015. Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia. PLoS One. 10(1): e0115168.

Yan L., Xiu, W.J, Jun, G.X. and Tong, C. 2011. The rps4 locus as an alternative marker for barcoding bryophytes: evaluation based on data mining from GenBank. Biodiversity Science. 19 (3): 311-318.

Yang, H.Q., An, M.Y., Gu, Z.J. and Tian, B. 2012. Genetic Diversity and Differentiation of Dendrocalamus membranaceus (Poaceae:

Bambusoideae), a declining bamboo species in Yunnan, China, as based on inter-simple sequence repeat (ISSR) analysis. International Journal of Molecular Science. 13: 4446-4457.

Yang, H.Q., Yang, J.B., Peng, Z.H., Gao, J., Yang, Y.M., Peng, S. and Li, D. Z. 2008. A molecular phylogenetic and fruit evolutionary analysis of the major groups of the paleotropical woody bamboos (Gramineae: Bambusoideae) based on nuclear ITS, GBSSI gene and plastid trnL-F DNA sequences. Molecular Phylogenetics and Evolution. 48: 809–824.

Yao, H., Song, J., Liu, C., Luo, K., Han, J., Li, Y., Pang, X., Xu, H., Zhu, Y., Xiao, P. and Chen, S. 2010. Use of ITS2 region as the universal barcode for plants and animals. PLoS One. 5 (10): e13102.

Yasodha, R. 2010. Characterization of microsatellite in the tribe Bambuseae. Gene Conserve. 10 (39): 51-64.

Zhang, Y.J., Ma, P.F. and Li, D.Z. 2011. High-throughput sequencing of six bamboo chloroplast genomes: phylogenetic implications for temperate woody bamboos (Poaceae: Bambusoideae). PLoS One. 6: e20596.

Zhang, Y.X., Xu, Y.X., Ma, P.F., Zhang, L.N. and Li, D.Z. 2013. Selection of potential plastid DNA barcodes for Bambusoideae (Poaceae). Plant Diversity and Resources. 35 (6): 743-750.

Zhao, H., Yang, L., Peng, Z., Sun, H., Yue, X., Lou, Y., Dong, L., Wang, L. and Gao, Z. 2015. Developing genome-wide microsatellite markers of bamboo and their applications on molecular marker assisted taxonomy for accessions in the

genus Phyllostachys. Scientific Reports: 5. Article number: 8018 doi: 10.1038/srep08018.

Zheng, C.Z., Zhang, Y.X., Triplett, J.K., Yang, J.B. and Li, D.Z. 2010. Large multilocus plastid phylogeny of the tribe Arundinarieae (Poaceae: Bambusoideae) reveals ten major lineages and low rate of molecular divergence. Molecular Phylogenetics and Evolution. 56: 821–839

Downloads

Published

07-11-2014

Issue

Section

Articles

How to Cite

DNA barcoding: An emerging tool for precise identification and certification of planting stock in taxonomically challenging bamboo species. (2014). Journal of Bamboo and Rattan, 13(1 & 2), 29-43. https://doi.org/10.55899/

Similar Articles

21-27 of 27

You may also start an advanced similarity search for this article.