Vol.16. Nos.4 (2017) pp.147-160
Title:

Meta-topolin overcomes seasonal dormancy and enhances in vitro axillary shoot proliferation in nodal explants of Pseudoxytenanthera ritcheyi – a commercially valuable bamboo

Authors:Vidya R. Sankar, E.M.Muralidharan

Abstract:The effect of selected parameters, which influence the establishment of in vitro shoot cultures through axillary bud proliferation from nodal explants of field grown clumps of Pseudoxytenanthera ritcheyi was studied. Single node explants collected across various seasons and cultured on a MS basal medium supplemented with various Plant Growth Regulators (PGRs) in CRD experiment. Explant size (diameter and length), position of the node on secondary branches as well as season of collection were important determinants of the time taken for frequency of axillary bud break as well as number and the length of shoots produced. Nodal explants of diameter 2-3 mm and 2.5-3 cm length were found to give the best results in all the parameters studied. Maximum frequency of bud break was obtained on 1 uM thidiazuron (TDZ) but shoot length was significantly reduced. The least effective PGR was Kinetin. BAP and meta-topolin gave similar results in the parameters studied, but the latter was superior in terms of its ability to overcome season-induced dormancy of buds. Incorporation of meta-topolin (10 μM) in culture media resulted in a consistently high axillary bud break throughout the year (86% to 98%).

Keywords:Pseudoxytenanthera ritcheyi, meta-topolin, bud break, multiple shoot formation, micropropagation

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