Authors:Wu, J.H; Wang, S.Y; Chang, S.T
Abstract:The purpose of this study is to establish a simple and reliable method for bamboo chlorophyll extraction. Chlorophylls in moso bamboo (Phyllostachys pubescens) epidermis were extracted with acetone, DMF (N,N-dimethylformamide) and DMSO (dimethyl sulfoxide) using three methods, including ultrasonics, centrifugation and grinding. Ultraviolet-visible spectrometry was then used to evaluate the efficiency of these extraction methods. It was also used to quantitatively analyze the extracted chlorophylls. The results revealed that the extraction efficiency of epidermis chlorophylls is related to the size of bamboo culm meal, solvent types, and the method of extraction and filtration. A fast and reliable extraction method was developed. It extracts chlorophylls from bamboo culm meal (
Keywords:Ultrasonics; chlorophyII